■ 基本信息
別名: | pAAV-RC2;pAAV-2 |
復(fù)制子: | pUC |
質(zhì)粒分類: | 腺相關(guān)病毒包裝質(zhì)粒 |
質(zhì)粒大小: | 7327bp |
原核抗性: | Amp |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
5'測序引物: | M13F:TGTAAAACGACGGCCAGT |
3'測序引物: | M13R:CAGGAAACAGCTATGACC |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞,腺相關(guān)病毒 |
質(zhì)粒用途: | 輔助包裝 |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標(biāo)記: |
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■ 質(zhì)粒簡介
pAAV-RC2是一個(gè)腺相關(guān)病毒包裝質(zhì)粒，攜帶了Cap和Rep基因，可與pAAV-MCS和pHelper質(zhì)粒一起包裝成腺相關(guān)病毒。
pAAV-MCS克隆質(zhì)粒：http://www.openstreetscalgary.com/shengwu_info.php?nid=2314&id=141
pAAV-RC2包裝質(zhì)粒：http://www.openstreetscalgary.com/shengwu_info.php?nid=2319&id=141
pHelper包裝質(zhì)粒：http://www.openstreetscalgary.com/shengwu_info.php?nid=2320&id=141
This 7.3 kb vector harbors the cap and rep genes that make the capsid and DNA replication proteins required to make infectious virions. This vector is cotransfected into the packaging cell line for production of recombinant, replication-deficient AAV virions.The pAAV-RC plasmid contains the AAV-2 rep and cap genes, encoding replication proteins and viral capsid structural proteins, respectively. Establishing the proper expression levels of rep and cap gene products is an important step in achieving high-titer virus production. pAAV-RC directs Rep and Cap expression from two different promoters, achieving optimal expression levels for each set of gene products.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK735pAAV-RC腺相關(guān)病毒包裝質(zhì)粒.txt
