■ 基本信息
啟動子: | T7 |
復制子: | p15A |
質粒分類: | 大腸桿菌載體；蛋白表達質粒 |
質粒大小: | 6706bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養條件: | 37℃，有氧，LB |
表達宿主: | 大腸桿菌 |
誘導方式: | IPTG或乳糖及其類似物 |
備注: | 溫控表達質粒 |
■ 質粒屬性
質粒宿主: | 大腸桿菌 |
質粒用途: | 蛋白表達 |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
|
熒光標記: |
|
■ 質粒簡介
pTXB1 contains a mini-intein from the Mycobacterium xenopi gyrA gene (Mxe GyrA intein; 198 amino acid residues) that has been modified to undergo thiol-induced cleavage at its N-terminus (3,5). The vector allows for the purification of a target protein without any extra amino acids by cloning into the NdeI and SapI sites. The target protein is fused at its C-terminus to a self-cleavable intein tag (~28 kDa) that contains the chitin binding domain (CBD, 6 kDa) allowing for affinity purification of the fusion precursor on a chitin column.
質粒只保證關鍵序列正確，不保證表達效果。
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK334pTXB1大腸表達質粒.txt
