■ 基本信息
別名: | PB513B-1；pB-CMV-MCS-ef1α-greenpuro |
啟動子: | CMV |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質粒分類: | 哺乳系列質粒；哺乳編輯質粒；哺乳轉座質粒 |
質粒大小: | 7258bp |
原核抗性: | Amp |
真核抗性: | Puro |
克隆菌株: | Stbl3 |
培養條件: | 37℃，5%CO2 |
表達宿主: | 293T等哺乳細胞 |
5'測序引物: | CMV-F（CGCAAATGGGCGGTAGGCGTG） |
3'測序引物: | Sv40-polyA-R（GAAATTTGTGATGCTATTGC） |
備注: | 哺乳細胞轉座表達載體 |
■ 質粒屬性
質粒宿主: | 哺乳細胞 |
質粒用途: | 蛋白表達,轉座 |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: | Puro
|
熒光標記: | 綠色
|
■ 質粒簡介
PiggyBac Dual promoter (PB513B-1)是用于哺乳細胞轉座實驗的質粒，位于CMV啟動子下游的多克隆位點（MCS），使目的基因或microRNA更方便克隆。下游的EF-1α core promoter啟動GFP的表達。
The PiggyBac (PB) transposon is a mobile genetic element that efficiently transposes between vectors and chromosomes via a "cut and paste" mechanism. During transposition, the PB transposase recognizes transposon-specific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and efficiently moves the contents from the original sites and efficiently integrates them into TTAA chromosomal sites. The powerful activity of the piggyBac transposon system enables genes of interest between the two ITRs in the PB vector to be easily mobilized into target genomes.
The unique features of piggyBac transposons are that there is NO Cargo Limit and it is also Reversible. Genomes containing an inserted piggyBac vector can be transiently re-transfected with the PB transposase expression vector. The PB transposase will remove the transposons from the genome, footprint-free.The Super PiggyBac transposase transient expression vector and PB513B-1 were co-transfected into HeLa cells and puromycin selection applied for 10 days (10ug/ml). Cells efficiently transposed were Puro resistant and GFP positive.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK564PiggyBac Dual promoter (PB513B-1)哺乳轉座質粒.txt
