■ 基本信息
別名: | pAAV-RC2;pAAV-2 |
復制子: | pUC |
質粒分類: | 腺相關病毒包裝質粒 |
質粒大小: | 7327bp |
原核抗性: | Amp |
克隆菌株: | Stbl3 |
培養條件: | 37度 |
表達宿主: | 哺乳細胞 |
5'測序引物: | M13F:TGTAAAACGACGGCCAGT |
3'測序引物: | M13R:CAGGAAACAGCTATGACC |
■ 質粒屬性
質粒宿主: | 哺乳細胞,腺相關病毒 |
質粒用途: | 輔助包裝 |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質粒簡介
pAAV-RC2是一個腺相關病毒包裝質粒，攜帶了Cap和Rep基因，可與pAAV-MCS和pHelper質粒一起包裝成腺相關病毒。
pAAV-MCS克隆質粒：http://www.openstreetscalgary.com/shengwu_info.php?nid=2314&id=141
pAAV-RC2包裝質粒：http://www.openstreetscalgary.com/shengwu_info.php?nid=2319&id=141
pHelper包裝質粒：http://www.openstreetscalgary.com/shengwu_info.php?nid=2320&id=141
This 7.3 kb vector harbors the cap and rep genes that make the capsid and DNA replication proteins required to make infectious virions. This vector is cotransfected into the packaging cell line for production of recombinant, replication-deficient AAV virions.The pAAV-RC plasmid contains the AAV-2 rep and cap genes, encoding replication proteins and viral capsid structural proteins, respectively. Establishing the proper expression levels of rep and cap gene products is an important step in achieving high-titer virus production. pAAV-RC directs Rep and Cap expression from two different promoters, achieving optimal expression levels for each set of gene products.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK735pAAV-RC腺相關病毒包裝質粒.txt
