■ 基本信息
啟動子: | CMV |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質粒分類: | 病毒系列，慢病毒克隆質粒 |
質粒大小: | 8102bp |
原核抗性: | Amp |
篩選標記: | Puro |
克隆菌株: | Stbl3 |
培養條件: | 37℃ |
表達宿主: | 哺乳細胞 |
誘導方式: | 無須誘導，瞬時表達 |
5'測序引物:
| CMV-F：CGCAAATGGGCGGTAGGCGTG
|
3'測序引物: | 根據序列設計引物 |
■ 質粒屬性
質粒宿主: | 哺乳細胞,慢病毒 |
質粒用途: | 蛋白表達 |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: | 嘌呤霉素
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熒光標記: |
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■ 質粒簡介
pLVX-Puro is an HIV-1-based, lentiviral expression vector. Lentiviral particles derived from the vector allow you to express your gene of interest in virtually any cell type, even primary cells. Expression of your gene is driven by the constitutively active human cytomegalovirus immediate early promoter (PCMV IE), located just upstream of the multiple cloning site (MCS), allowing constitutive, high level expression of your protein of interest.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK756pLVX-Puro慢病毒表達質粒.txt
