■ 基本信息
啟動子: | H1 promoter |
復制子: | pBR322 ori，SV40 ori |
質粒大小: | 4555bp |
原核抗性: | Amp |
真核抗性: | Hyg |
克隆菌株: | Stbl3 |
培養條件: | 37度 |
表達宿主: | 哺乳細胞 |
■ 質粒屬性
質粒宿主: | 哺乳細胞 |
質粒用途: | 基因沉默 |
片段類型: | shRNA
|
片段物種: |
|
原核抗性: | Amp |
真核抗性: | Hyg
|
熒光標記: |
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■ 質粒簡介
pSilencer3.1-H1-Stuffer-Hygro是一個哺乳細胞干擾載體，通過BamHI和HindIII酶切位點可連入SiRNA，點擊查看本質粒的參考資料：
pSilencer hygro Kit.pdf.（點擊查看）
The pSilencer vectors employ RNA polymerase III (pol III) promoters which generate large amounts of small RNA using relatively simple promoter and terminator sequences. They also include an antibiotic resistance gene that provides a mechanism to select for transfected cells that express the introduced DNA.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK1717 pSilencer3.1-H1-Stuffer-Hygro哺乳干擾質粒.txt
